Synthetic lipopeptide vaccines with B-, T-helper- and T-killer cell response and built in adjuvanticity

The N-terminus of bacterial lipoprotein tripalmitoyl-S-glyceryl-cysteinyl-serine (P3CS) has been synthesized for the first time in our laboratory.  P3CS and  water –soluble lipopeptides such as P3CSK4  proved to be powerful immunoadjuvants activating Toll-like receptors TLR 1,2. The conjugation of P3CS with species- and allele-specific B-, T-helper- and CTL-epitopes allowed us to prepare chemically defined, complete minimal lipopeptide vaccines with molecular masses below 5000. The synthetic lipopeptides allowed for the first time the in vivo priming of cytotoxic T-lymphocytes and antibody formation.

Lipopeptides are available at EMC microcollections.

Synthetic adjuvant Pam3Cys-Ser-(Lys)4

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