B- and T-cell epitope mapping of viral proteins

Multiple parallel peptide synthesis has been optimized for the production of thousands of single analytically characterized peptides. The peptides were used for epitope fine mapping of complete viral protein sequences (HIV, hepatitis C, measles, etc.). Localized B-cell epitopes were conjugated with biotin to allow the ELISA diagnostics and with lipopeptide (P₃CS) to produce antibodies. The epitope mapping has been supported by conformational mapping using circular dichroism (CD) and conformational predictions. In parallel a complete T-cell epitope mapping (T-helper and CTL) was performed.

Di – to heptapeptide mapping of the binding region of HIV-1 Nef-protein to MAB Dü6

F. O. Gombert, W. Blecha, M. Tähtinen, A. Ranki, S. Pfeiffer, W. Tröger, R. Braun, K.
Müller-Lantzsch, G. Jung, H. Rübsamen-Waigmann, and K. Krohn (1990). Antigenic Epitopes of NEF Proteins from Different HIV-l Strains as Recognized by Sera from Patients with Manifest and Latent HIV Infection, Virology 176, 458-466.

W. van’t Hoff, P. C. Driedijk, M. van den Berg, A. G. Beck-Sickinger, G. Jung, and R.
C. Aalberse (1991). Epitope Mapping of the Dermatophagoides pteronyssinus House Dust Mite Major’ Allergen Der p II Using Overlapping Synthetic Peptides, Mol. Irnrnunol. 28, 1225-1232.

N. Zimmermann, A.G. Beck-Sickinger, G. Folkers, S. Krickl, I. Müller, and G. Jung
(1991). Conformational and Epitope Mapping of Herpes simplex Virus Type 1 Thymidine
Kinase Using Synthetic Peptide Segments, Eur. J. Biochern. 200,519-528.

M. Tähtinen, F. Gombert, E.-R. Hyytinen, G. Jung, A. Ranki, and K J. E. Krohn (1992).
Fine Specificity of the B Cell Epitopes Recognized in HIV-l Nef by Human Sera.
Virology 187, 156-164.

J.-P. Borg, H.G. Ihlenfeldt, G. Jung, G. Haas, and M. Pierres (1994). HIV-l Reverse Transcriptase Immunodominant CD4+ T Cell Epitopes: A Peptide-Based Multiparametric Assessment in Mouse, Eur. J. Immunol. 24,1496-1502.

C.P. Muller, R. Bünder, H. Mayser, S. Ammon, M. Weinmann, N.H.C. Brons, F.
Schneider, G. Jung, and K.-H. Wiesmüller (1995). Intramolecular Immunodominance and Intermolecular Selection of H2^d – Restricted Peptides Define the Same Immunodominant Region of the Measles Virus Fusion Protein, Mol. Immunology 32, 37-47.

E. Armengol, K.-H. Wiesmüller, G. Jung, and A. Saalmüller (2002). Identification of T-Cell Epitopes on Structural and Non-Structural Proteins of Classical Swine Fever Virus, J. Gen. Virology 83, 551-560.