Combinatorial peptide libraries in assays to determine exact MHC-binding motifs, T-cell receptor recognition and TAP transporter

Combinatorial linear peptide libraries X8 to X15 with defined sequence positions are highly complex peptide pools. Pool sequencing and MS analyses allowed their characterization. The peptide libraries are excellent tools to determine the amino acid residues responsible for binding of peptides to MHC I and II. T-cell recognition of peptide-MHC-complexes can be perfectly mapped. The recognition of peptides by the transporter associated with antigen processing (TAP) was studied in detail.  Independently from the studies of natural ligands our libraries allow the determination of a valuable molecular basis for the development of novel assays and immunotherapeutics, and for the understanding of protein-peptide interactions in general.

Allele-specific activity pattern of undecapeptide collections


K.-H. Wiesmüller, M. Brich, G. Jung, K. Sparbier, and P. WaIden (1995). Peptide Binding to MHC Class I Molecules Analyzed by Confocal Microscopy, Eur. J. Cell Biol. 66, 389-393.

K. Udaka, K.-H. Wiesmüller, S. KienIe, G. Jung, and P. Waiden (1995). Decrypting the Structure of Major Histocompatibility Complex Class I-Restricted Cytotoxic T Lymphocyte Epitopes with Complex Peptide Libraries, J.Exp.Med. 181,2097-2108.

S. UebeI, T.H. Meyer, W. Kraas, S. Kienle, G. Jung, K.-H. Wiesmüller and R. Tampé
(1995). Requirements for Peptide Binding to the Human Transporter Associated with An-
tigen Processing Revealed by Peptide Scans and Complex Peptide Libraries,
Biol. Chem.
31, 19512-18516.

K. Udaka, K.-H. Wiesmüller, S. Kienle, G. Jung, and P. Walden (1995). Tolerance to Amino Acid Variations in Peptides Binding to the Major Histocompatibility Complex Class I Protein H-2Kb, J. Biol. Chem. 270, 24130-24134.

B.R. Gundlach, K.-H. Wiesmüller, T. Junt, S. KienIe,.G. Jung, and P. Waiden (1996).
Specificity and Degeneracy of Minor Histocompatibility Antigen-Specific MHC-
Restricted CTL,
J. lmmunol. 156, 3645-3651.

S.Uebel, W. Kraas, S. Kienle, K.-H. Wiesmüller, G. Jung, and R. Tampé (1997). Recognition Principle of the TAP Transporter Disclosed by Combinatorial Peptide Libraries, Proc. Natl. Acad. Sci. USA 94, 8976-8981.

D.G. Schmid, F. v.d. Mülbe, B. Fleckenstein, T. Weinschenk, and G. Jung (2001).
Broadband Detection Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry to Reveal Enzymatically and Chemically Induced Deamidation Reactions within Peptides, Anal. Chem. 73, 6008-6013.

 B. Fleckenstein,  Ø. Molberg, S. Qiao, D.G. Schmid, F. von der Mülbe, K. Elgstøen, G. Jung, and L. M. Sollid (2002). Gliadin T Cell Epitope Selection by Tissue Transglutaminase in Celiac Disease: Role of Enzyme Specificity and pH Influence on Transamidation vs. Deamidation Reaction, J. Biol. Chem. 277, 34109-34116.

B. Fleckenstein, S.-W. Qiao, M.R. Larsen, G. Jung, P. Roepstorff, and L.M. Sollid (2004).
Molecular Characterization of Covalent Complexes Between Tissue Transglu-taminase and Gliadin Peptides, J. Biol. Chem. 279, 17607-17616.

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